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Evolution at the scene of the crime
March 2006, updated August 2012

comparing DNA samples
When forensic scientists examine DNA in the lab, each sample appears as a unique sequence of dark bars. Patterns of bars are compared to find a match. In the hypothetical example shown here, it looks like suspect #2 left some DNA at the crime scene.
The tests confirm, beyond a shadow of doubt, that Roger Keith Coleman did it, but Alan Crotzer did not. In 1992, Coleman was executed for the rape and murder of his sister-in-law. In 1981, Crotzer was sentenced to 130 years in prison for a robbery and pair of rapes. Though the crimes themselves are old, judgments long since rendered, and punishments already meted out, for many observers, the actual guilt or innocence of these two defendants for two different crimes was only just settled by an increasingly important test: the DNA fingerprint. Recent DNA tests revealed that it was, indeed, Coleman's semen in the body of his victim, and that he had actually committed the crime for which he was executed more than 10 years ago. And recent DNA tests revealed that Crotzer is not a rapist and has spent 24 years in prison for crimes he did not commit.

Where's the evolution?
DNA fingerprinting allows forensic scientists to determine whether the DNA found at a crime scene came from a particular individual. But how does this technique work and what does it have to do with evolution? Answering this question depends upon understanding the genetic variation in human populations and the rates at which different parts of the genome evolve.

Humans are 96% genetically identical to our closest living relative, the chimpanzee. Obviously, we are even more similar to each other: two randomly chosen people from anywhere on Earth are expected be 99.9% genetically identical. So how can one person's DNA be unique enough to identify him or her as the perpetrator of a particular crime? The answer, it turns out, is volume: the human genome is composed of three billion base pairs! Even at 99.9% similarity, any two people will still differ at about three million base pairs. In fact, no two people on Earth have exactly the same genetic sequence, except identical twins.

Some of these genetic differences influence the unique set of characteristics that makes you you: eye color, hair color, height, tendency towards heart disease, and numerous other traits. But most of these genetic differences have no discernable effect on your phenotype, or set of physical features, at all. And it is these genetic differences that biologists focus on when they are trying to identify or exonerate a suspect using DNA fingerprinting.

Different parts of the genome evolve at different rates. DNA that encodes important traits tends to evolve slowly. This is because most (though not all) mutations in critical regions of the genome are likely to cause detrimental effects and be selected out of the population rapidly. If a stretch of DNA evolves slowly, few changes in its sequence will occur and many people in the population will likely carry identical sequences. Though important, these regions of the genome will not be very helpful for identification.

On the other hand, some regions of the genome don't seem to do anything in particular. Because variation in these regions has little effect on the characteristics of the organism, variants are largely "invisible" to natural selection. Here, mutations accumulate without much consequence and gene frequencies change via genetic drift. These regions evolve quickly, and as a consequence, different individuals in the population carry different sequences in these regions.

Even within fast-evolving regions of the genome, there may be particular mutation "hotspots," which are unusually variable in sequence. Many of these regions contain DNA that repeats the same sequence of bases over and over again (e.g., ATGGATGGATGGATGG...). Biologists think that cells frequently make mistakes copying these regions, accidentally producing more or fewer repeats than in the original DNA sequence and, hence, causing a new mutation. Because they evolve so quickly and vary so much in the number of repeats, these hotspots are ideal targets for DNA fingerprinting.

In DNA fingerprinting, scientists collect samples of DNA from different sources — for example, from a hair left behind at the crime scene and from the blood of victims and suspects (see diagram below). They then narrow in on the stretches of repetitive DNA scattered throughout these samples. The profile of repetitive regions in a particular sample represents its DNA fingerprint, which ends up looking a bit like a barcode. Each bar in the barcode represents one particular stretch of repetitive DNA. Since these repetitive regions are common in the genome and highly variable from individual to individual, no two people (except identical twins) will have exactly the same set of repetitive regions and, hence, the same DNA fingerprint.

comparing lengths of repeating DNA

Download this graphic from the Image library.

The importance of DNA fingerprinting for figuring out who was involved in a particular crime is clear: since the advent of the technique, DNA evidence has exonerated more than 150 wrongly convicted people and has become an accepted and expected line of evidence in many thousands of trials. The approach has other applications as well, including determining family relations (such as in paternity suits) and helping biologists study mating habits in the wild. However, it's important to keep in mind that the technique only works because evolution does: the human genome is constantly evolving, acquiring new mutations over time — and it is the variation generated by this evolution that forensic scientists leverage to help solve crimes.


News update, August 2012

In 2006, we explained how evolutionary history allows DNA fingerprinting to be used to catch criminals. Now, doubts are being raised about an extension of this technique called familial searching. In standard fingerprinting, forensic scientists look for an exact match between crime scene DNA and that of a suspect or past offender. No problems there. However, if an exact match is not found, in some states, familial searching will be used. In this case, the criminal database is searched for a partial match, which might indicate that the perpetrator is a close relative of a known criminal. The key word here is might. Earlier this year, scientists from University of Washington and UC Berkeley showed that this practice can lead to racial bias. An evolutionary perspective helps us understand why ...

People with recent shared evolutionary ancestry (e.g., the same ethnicity) are likely to have similar genetic sequences. For example, two people of Vietnamese descent are more likely to both have a sequence of 49 'GT' repeats in a particular spot in the genome than are two people of different ethnic backgrounds. You might think that this causes a lot of people to be falsely accused of crimes, but it does not because forensic scientists examine at least 10 different regions of the genome, not just one. These different regions are standardized and were selected because of their ability to discriminate among different individuals. People with the same ethnic background might match at some of these spots, but are astronomically unlikely to match at all 10 (unless they are identical twins). However, familial searching involves looking for partial matches. Will two people of a particular ethnicity (e.g., a criminal and an unrelated individual who both happen to be Vietnamese) match at five different regions of the genome? The answer depends on which ethnic group the people belong to, the new research suggests. The genome regions involved in criminal DNA fingerprinting were selected based on genetic sequences from people of African American, European American, Southeastern Latino, and Southwestern Latino descent. But other ethnic groups were not studied for this purpose. These groups may have low variability in these regions (or low variability overall), and so unrelated individuals may be more likely to match. The problem is further exacerbated if these groups are already overrepresented in criminal databases. Because of these issues familial searching is likely to cause people of Native American and Asian descent to be wrongfully investigated at alarmingly high rates.

Currently, familial searching is used by California, Virginia, and Colorado. Though it may allow law enforcement to identify criminals in some difficult cases, adoption of the technique has been slow because of concerns over privacy and racial bias.



Read more about it

Primary literature:

  • Jeffreys, A.J., Wilson, V., and Thein, S.L. (1985). Individual-specific 'fingerprints' of human DNA. Nature 316(6023):76-79.
    read it

  • Jeffreys, A.J., Brookfield, J.F.Y., and Semeonoff, R. (1985). Positive identification of an immigration test-case using human DNA fingerprints. Nature 317:818-819.
    read it

  • Rohlfs, R. V., Fullerton, S. M., and Weir, B. S. (2012). Familial identification: population structure and relationship distinguishability. PloS Genetics. 8: e1002469.
    read it


News articles:

Understanding Evolution resources:

Discussion and extension questions

  1. What is a phenotype? Describe two ways in which the sequence of your DNA might affect your phenotype. Why don't the repetitive sequences described in the article seem to affect the carrier's phenotype?

  2. What is a mutation?

  3. Do all parts of the genome experience mutation at the same rate? List some factors that affect rate of mutation and describe how they affect it.

  4. What does each "bar" in a DNA fingerprint represent?

  5. Why can't identical twins be distinguished from each other using DNA fingerprinting?

  6. Mutation is critical to the processes of evolution. Read about genetic variation and natural selection, and then explain why mutation is so important in evolution.


Related lessons and teaching resources

  • Teach about DNA fingerprinting: This online activity for grades 9-12 teaches the basics of DNA fingerprinting as students solve a crime. Related lessons are included in the Teaching Activities section of the site.

  • Teach about other high-tech applications of evolution: In this article for AP biology students or undergraduates, evolutionary biologist Jim Bull gives his perspective on how evolution matters to society today. Related lessons are included in the Education Resources section of the site.


References

  • DNA: Virginia executed the right man. (2006, January 12). CNN.
    Retrieved January 26, 2006 from CNN

  • Handwerk, B. (2005, April 8). DNA frees death-row inmates, brings others to justice. National Geographic Channel.
    Retrieved January 26, 2006 from National Geographic

  • Innocence Project. (n.d.) Innocence project: Case profiles.
    Retrieved January 26, 2006 from Innocence Project

  • Jeffreys, A.J., Wilson, V., and Thein, S.L. (1985). Individual-specific 'fingerprints' of human DNA. Nature 316(6023):76-79.
    read it

  • Jeffreys, A.J., Brookfield, J.F.Y., and Semeonoff, R. (1985). Positive identification of an immigration test-case using human DNA fingerprints. Nature 317:818-819.
    read it

  • Man exonerated by DNA walks free after 24 years. (2006, January 23). USA Today.
    Retrieved January 26, 2006 from USA Today

  • Mindell, D. P. (2005). Evolution helps solve crimes. In J. Cracraft & R. W. Bybee (Eds.), Evolutionary Science and Society: Educating a New Generation. (pp. 173-177). Washington DC: American Institute of Biological Sciences.

  • National Human Genome Research Institute. (n.d.) Human genetic variation: Teacher's guide.
    Retrieved January 26, 2006 from National Institutes of Health

  • National Human Genome Research Institute. (2005, August 31). New genome comparison finds chimps, humans very similar at the DNA level. NIH News.
    Retrieved January 26, 2006 from National Human Genome Research Institute

  • Rohlfs, R. V., Fullerton, S. M., and Weir, B. S. (2012). Familial identification: population structure and relationship distinguishability. PloS Genetics. 8: e1002469.
    read it




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